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PCR and Real-Time PCR


Description

Clinical diagnosis has made extensive use of molecular techniques, including disease diagnosis, disease prognosis, and identification of infectious agents. The concepts of molecular biology laboratory practise, common techniques, and the scientific foundation for molecular diagnostics will all be covered in this fundamental molecular biology course series. This introductory-level eLearning course explains the real-time PCR and PCR concept. Real-time PCR features, PCR product analysis, and PCR processes are some of the subjects discussed.

Topics to be covered

At the conclusion of this course, the participant will be able to:

• Explain the basic steps involved in PCR

• Identify the components of PCR, reverse transcription PCR, and PCR product analysis

• Recognize the characteristics of real-time PCR

• Identify the techniques used to detect products in real-time PCR

• Differentiate the nucleic acid quantification processes used in real-time PCR

• Explain the roles of PCR controls

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