Background introduction The full name of the EWSR1 gene is Ewing sarcoma breakpoint region 1 gene. First discovered in Ewing's sarcoma, located at 22q12, consisting of 17 exons, encoding a nuclear protein of 656 amino acids. It is an RNA binding protein. It plays an important role in mitotic cell separation, spindle formation, microtubule stability, DNA repair and cell senescence. It belongs to the cytokine TET family members, which controls cell growth. Probe description EWSR1 gene break apart probe uses orange dye to label the 5'end region of EWSR1 gene and green dye to label the 3'end region of EWSR1 gene. EWSR1 gene break apart probe can detect all EWSR1 gene rearrangements. Clinical significance EWSR1 gene family members have TLS/FUS and TAFl5 genes, all of which are involved in gene translocation of various soft tissue sarcomas, and are fused with transcription factor genes containing the DNA binding domain to form new fusion transcription factors with obvious Tumorigenic effect. Detecting whether EWSR1 gene is broken or not, can be used as auxiliary diagnosis basis for Ewing's sarcoma family tumor.
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EWSR1 gene detection probe kit
MDM2 gene amplification detection probe kit
Background introduction MDM2 gene is located in the q15 region of chromosome 12, and the encoded P90 protein can bind to P53 gene, causing P53 gene to lose its normal function, leading to tumorigenesis. Probe description MDM2 gene amplification probe uses an orange-red dye to label the MDM2 gene region, and a green chromosome to label chromosome 12 centromere region (CEP12). MDM2 gene marker region is located at 12q15, and the CEP12 probe adopts an alpha satellite sequence, which has extremely high specificity and does not hybridize with other chromosome centromeres to produce noisy spots.
Clinical significance
MDM2 gene amplification is the most common abnormality in fibrosarcoma and can assist in the diagnosis of fibrosarcoma; this gene amplification also occurs in osteosarcoma (16%) and esophageal cancer (13%). Used to guide the treatment of MDM2 inhibitors.
SYT (SS18) gene break apart probe detection kit
Background introduction SYT (SS18) gene is located in the q11.2 region of chromosome 18 and encodes a transcriptional co-activator. Specific SYT (SS18) gene translocation exists in 90% of synovial sarcomas. Probe description SYT (SS18) gene break apart probe uses an orange dye to label the 5'end region of SYT (SS18) gene and a green dye to label the 3'end region of SYT (SS18) gene. SYT (SS18) gene break apart probe can detect all SYT (SS18) gene rearrangements.
Clinical significance
Specific chromosomal translocation t (X:18) was found in 90% of patients with synovial sarcoma (p11.2: q11.2). This translocation results in the fusion of the SYT (SS18) gene on chromosome 18 with the SSX1 or SSXE gene on the X chromosome. This is used to assist in the diagnosis of synovial sarcoma.